Anti-Muscarinic Acetylcholine Receptor (M1) antibody produced in rabbit
SIGMA/M9808 - affinity isolated antibody, lyophilized powder
Synonym: Acetylcholine Receptor antibody
MDL Number: MFCD02096983
Product Type: Chemical
Immunocytochemistry JOURNAL CITATION: M1 muscarinic receptor activation mediates cell death in M1-HEK293 cells. By: Graham, E. S., Woo, K. K., et al. in PLoS One, 2013. PubMed ID: 24023725 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0072011), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Anti-M1 Muscarinic Receptor : Cat. No. M9808: Immunohistochemical staining of Rat striatum (ST) using Anti-M1 Muscarinic Receptor antibody. A. M1 Muscarinic Receptor appears in the striatum (green). B. Staining of interneurons with Mouse Anti-Parvalbumin (PV, red). C. Confocal merge of M1 Muscarinic Receptor and PV demonstrates localization of PV expressing neurons in the striatal matrix; not in striatal patches (P).
Immunohistochemistry Anti-M1 Muscarinic Receptor : Cat. No. M9808: Immunohistochemical staining of Mouse striatum (ST) using Anti-M1 Muscarinic Receptor antibody. A. M1 Muscarinic Receptor appears in the striatum (green). B. Staining of interneurons with Mouse Anti-Parvalbumin (PV, red). C. Confocal merge of M1 Muscarinic Receptor and PV demonstrates localization of PV expressing neurons in the striatal matrix; not in striatal patches (P).
Immunoblotting Anti-M1 Muscarinic Receptor: Cat. No. M9808: Western blot analysis of Rat brain membranes. Lanes 1. Anti-M1 Muscarinic Receptor antibody, (1:200). 2. Anti-M1 Muscarinic Receptor antibody, preincubated with the control fusion protein antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1:200 using rat brain membranes |
| immunohistochemistry: suitable | |
| UniProt accession no. | P08482  |
| Application: | Anti-Muscarinic acetylcholine receptor (M1) antibody produced in rabbit has been used in immunohistochemistry and Western blotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Biochem/physiol Actions: | Muscarinic acetylcholine receptor 1 (M1) regulates normal prostate growth and reduced-level glandular secretion. Muscarinic receptors play a major role in tumor progression. Muscarinic acetylcholine receptors (mAChRs) regulates adenylyl cyclase and potassium channel activities. It also stimulates various mitogen-activated protein kinases. M1 may be useful for the discovery of therapeutics for Alzheimer′s disease. |
| Biochem/physiol Actions: | Studies have shown that activation of muscarinic cholinergic receptor 1 (M1) in rats leads to changes in the endotoxin tolerance in the liver. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Muscarinic acetylcholine receptor 1 (M1) is a member of class A G protein-coupled receptors (GPCRs) family, which is encoded by the CHRM1 gene. Muscarinic receptors have seven transmembrane domains localized in the parasympathetic nerve sub-synaptic membrane. The CHRM1 gene is located on human chromosome location 11q12.3. |
| General description: | Muscarinic cholinergic receptor 1 (M1) is seen in the forebrain of rats. |
| Immunogen: | GST fusion protein of a part of the i3 intracellular loop of human M1 muscarinic acetylcholine receptor (mAChR) corresponding to amino acid residues 227-353.1, 2 |
| Physical form: | Lyophilized from a solution of phosphate-buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |