Monoclonal Anti-MAP2 antibody produced in mouse
SIGMA/M9942 - clone HM-2, purified from hybridoma cell culture
MDL Number: MFCD00164683
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus. By: Wilson, N. M., Titus, D. J., et al. in Front Syst Neurosci, 2016. PubMed ID: 26903822 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/Article/10.3389/fnsys.2016.00005/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus. By: Wilson, N. M., Titus, D. J., et al. in Front Syst Neurosci, 2016. PubMed ID: 26903822 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/Article/10.3389/fnsys.2016.00005/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus. By: Wilson, N. M., Titus, D. J., et al. in Front Syst Neurosci, 2016. PubMed ID: 26903822 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/Article/10.3389/fnsys.2016.00005/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence B35 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 2 μg/mL Monoclonal Anti-MAP2 Clone: HM-2 (Cat. No. M9942). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 (Cat. No. M9942). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBTBCIP substrate. Antibody concentration: 1 μg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HM-2, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~280 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, chicken, human, mouse, bovine, quail |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using rat brain extract | |
| UniProt accession no. | P11137  |
| Application: | Anti-MAP2 antibody has been used in immunofluorescence. It has also been used in immunohistochemistry. |
| Application: | May be used in various immunochemical techniques including immunoblotting, immunohistochemistry, and immunocytochemistry. Monoclonals antibodies to MAP2 are used to study the cytoskeleton structure in cells. By immunoblotting, a working antibody concentration of 1-2 μg/ml is recommended using a rat brain extract. |
| Biochem/physiol Actions: | MAP2 (microtubule-associated protein 2) expression is monitored to predict the disease progression in glioma. MAP2 is critical in the formation of dendritic protrusions in astrocytes and oligodendrocytes. In eukaryotes, MAP2 is essential for spindle assembly and chromosome segregation. |
| Biochem/physiol Actions: | Reacts with all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. Does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain. |
| Biochem/physiol Actions: | This antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280 kDa (MAP2a and b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and neurons have reached their mature morphology. MAP2 is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton. |
| Immunogen: | rat brain microtubule-associated proteins (MAPs). |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 C for up to one month. For extended storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |