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ALT Activity Assay

SIGMA/MAK052 - sufficient for 100 colorimetric or fluorometric tests

Synonym: Alanine Aminotransferase Activity Assay Kit; SGPT Activity Assay Kit; Serum Glutamic-Pyruvic Transaminase Activity Assay Kit

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-MAK052-1KT 1 kit
 DISCONTINUED  
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
ALT Activity Assay standard curve.
ALT Activity Assay positive control.

 

detection method colorimetric
  fluorometric
relevant disease(s) gastrointestinal diseases
shipped in wet ice
storage temp. −20°C
usage sufficient for 100 colorimetric or fluorometric tests
Application: ALT Activity Assay kit has been used to determine the activity of alanine aminotransferase (ALT) in serum samples.
Application: Suitable for the measurement of alanine aminotransferase (ALT) activity in biological samples including tissue, cells and serum.
Biochem/physiol Actions: The ALT Activity Assay Kit provides a simple and direct procedure for measuring ALT activity in a variety of biological samples.

ALT activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate generated. One unit of ALT is defined as the amount of enzyme that generates 1.0 μmole of pyruvate per minute at 37 °C.
Features and Benefits: Compatible with high-throughput handling systems.
General description: Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), is a pyridoxal-phosphate-dependent enzyme that catalyzes the reversible transfer of an amino group from alanine to α-ketoglutarate, generating pyruvate and glutamate. ALT is found primarily in liver and serum, but occurs in other tissues as well. Hepatocellular injury often results in an increase of serum ALT levels and serum ALT levels can be used as a marker for liver injury.
Storage Temp. −20°C
UNSPSC 12161503

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