Glyoxalase I Activity Assay Kit
SIGMA/MAK114 - sufficient for 100 tests (UV)
Synonym: Aldoketomutase Activity Assay; GLO1 Activity Assay; Ketone-Aldehyde Mutase, Activity Assa; Lactoylglutathione Lyase Activity Assay; Methylglyoxalase Activity Assay
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| detection method | colorimetric |
| relevant disease(s) | cancer |
| storage temp. | −20°C |
| usage | sufficient for 100 tests (UV) |
| Application: | Glyoxalase I Assay Kit has been used to estimate the glyoxalase I activity in plasma samples. |
| Application: | Suitable for the detection of Glyoxalase I activity in enzyme preparations and biological samples. Suitable for testing the effects of compounds on Glyoxalase I activity. |
| Biochem/physiol Actions: | The Glyoxalase I Activity Assay kit provides a simple and direct procedure for measuring GLO-1 activity in a variety of samples such as enzyme preparations or biological samples. In this assay, the GLO-1-mediated production of S-lactoylglutathione is measured by monitoring the change in absorbance at 240 nm. One unit of Glyoxalase I is the amount of enzyme that will convert 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.6 and 25 °C. |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Glyoxalase I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methyglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans, and cancer. |
| Symbol |   GHS07,GHS08 |
| Signal word | Warning |
| Hazard statements | H317 - H341 |
| Precautionary statements | P201 - P202 - P261 - P280 - P302 + P352 - P308 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |