Phospholipid Assay Kit
SIGMA/MAK122 - sufficient for 100 colorimetric or fluorometric tests
Synonym: Phospholipid Quantification Kit
Product Type: Chemical
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| application(s) | cosmetics food and beverages pharmaceutical |
| detection method | colorimetric |
| fluorometric | |
| shipped in | dry ice |
| storage temp. | −20°C |
| usage | sufficient for 100 colorimetric or fluor |
| Application: | Phospholipid assay kit has been used in biochemical analysis and phospholipid quantitation. |
| Application: | Suitable for the detection of phospholipids in biological samples |
| Biochem/physiol Actions: | In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays. |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P304 + P340 + P312 |
| Hazard Codes | Xi |
| Risk Statements | 36-52/53 |
| Safety Statements | 26-61 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |