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β-Glucosidase Activity Assay Kit

SIGMA/MAK129 - sufficient for 100 colorimetric tests

Synonym: Beta-Glucosidase Assay Kit

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-MAK129-1KT 1 kit
$645.00
1/EA
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detection method colorimetric
relevant disease(s) neurological disorders; gastrointestinal diseases; hematological disorder
storage temp. −20°C
usage sufficient for 100 colorimetric tests
Application: The β-Glucosidase Activity Assay kit provides a simple and direct procedure for measuring β-glucosidase activity in biological samples, for characterizing β-glucosidase production, and for the screening of β-glucosidase modulators.
Application: β-Glucosidase Activity Assay Kit has been used to measure the β-glucosidase activity of the sample.
Biochem/physiol Actions: In this assay, β-glucosidase activity is determined by a reaction in which β-glucosidase hydrolyzes p-nitrophenyl-β-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the β-glucosidase activity present. One unit of β-Glucosidse is the amount of enzyme that catalyzes the hydrolysis of 1.0 μmole substrate per minute at pH 7.0.
Features and Benefits: Compatible with high-throughput handling systems.
General description: β-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing b(1→4)-linked D-glucose residues with the release of D-glucose. β-Glucosidases are required by organisms for the consumption of cellulose. Lysozyme, a β-glucosidase present in tears, acts on the β(1→4) glucose bonds present in the peptidoglycan cell wall of Gram-negative bacteria and helps to prevent bacterial infections in the eye. Defects in β-glucosidase activity have been implicated in Gaucher′s disease and Parkinson′s disease.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Storage Temp. −20°C
UNSPSC 12161503

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