ADP Assay Kit
SIGMA/MAK133 - sufficient for 100 assays (bioluminescent)
Product Type: Chemical
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| detection method | chemiluminescent |
| relevant disease(s) | hematological disorder; cancer |
| storage temp. | −20°C |
| usage | sufficient for 100 assays (bioluminescent) |
| Application: | Suitable for the detection of ADP in cells, tissue and other biological samples. |
| Biochem/physiol Actions: | The ADP Assay kit provides a simple and direct procedure for measuring ADP levels in cells and other biological samples. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presences of luciferse, ATP immediately reacts with the substrate ( Luciferase ATP + In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the |
| General description: | Adenosine diphosphate (ADP) is a nucleoside that plays a critical role in energy transfer reactions. ADP is produced from adenosine triphosphate via the action of ATPases. ADP also plays a critical role in platelet function. ADP, stored in plate-dense granules, is released upon platelet activation where it acts on purinergic receptors to mediate intracellular signaling and platelet aggregation. The ADP Assay kit provides a simple and direct procedure for measuring ADP levels in cells and other biological samples. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presences of luciferse, ATP immediately reacts with the Substrate (D-luciferin) to produce light. The light intensity is a direct measure of the intracellular ATP concentration and is stable over several minutes. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn,N |
| Risk Statements | 20/21/22-36-43-51/53 |
| Safety Statements | 26-36/37-61 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |