Autophagy Assay Kit
SIGMA/MAK138 - sufficient for 200 fluorometric tests
Product Type: Chemical
Autophagy Assay Kit, Cat. No. MAK138: Autophagy Blue labeled vesicles are induced by starvation in HeLa cells. HeLa cells were incubated in a regular DMEM medium as control (A) or in a serum-depleted medium as autophagy treatment (B) for 16 hours. Both control cells and starved cells were incubated with Autophagy Blue working solution for 30 minutes in a 37 °C, 5% CO2 incubator, and then washed four times with wash buffer. Cells were imaged immediately under a fluorescence microscope with a DAPI channel. Autophagy is indicated by bright blue dot staining of autophagic vacuoles (B).
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| detection method | fluorometric |
| relevant disease(s) | cancer; immunological diseases |
| storage temp. | −20°C |
| usage | sufficient for 200 fluorometric tests |
| Application: |
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| Application: | This kit is suitable for the detection of autophagy in mammalian cells by fluorescence microscopy and fluorescence microplate readers. |
| Biochem/physiol Actions: | The Autophagy Assay kit provides a simple and direct procedure for measuring autophagy in a variety of cell types using a proprietary fluorescent autophagosome marker (λex = 333/λem = 518 nm). |
| General description: | Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway. Activation of the autophagy pathway is required for multiple cellular roles, including survival during starvation, the clearance of intracellular components, development, and immunity. |
).| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |