Fluorometric Intracellular Ros Kit
SIGMA/MAK143 - sufficient for 200 fluorometric tests (green)
Synonym: Fluorometric ROS Detection Kit
Product Type: Chemical
Fluorometric Intracellular Ros Kit, Cat. No. MAK143: Detection of ROS in Jurkat cells. Jurkat cells were seeded on the same day at 300,000 cells/100 μL/well in a black wall/clear bottom 96-well plate. The ROS assay loading solution (100 μL/well) was added and incubated in a 5% CO2, 37 °C incubator for 1 hour. And then the cells were treated with 1 mM, 0.1 mM H2O2 or without H2O2 for 30 minutes. The fluorescence signal was monitored at Ex/Em = 490/525 nm (cutoff at 515 nm) with bottom read mode.
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| detection method | fluorometric |
| relevant disease(s) | cancer; neurological disorders |
| storage temp. | −20°C |
| usage | sufficient for 200 fluorometric tests (green) |
| Application: | Fluorometric Intracellular Ros Kit has been used in reactive oxygen species (ROS) generation assay. |
| Application: | This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope. |
| Biochem/physiol Actions: | The Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product (λex = 490/λem = 520 nm) proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and read using a fluorescence microscope or a fluorescence microplate reader. |
| General description: | Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |