Fluorometric Intracellular Ros Kit
SIGMA/MAK145 - sufficient for 200 fluorometric tests (red)
Synonym: Intracellular ROS Fluorescence Assay
Product Type: Chemical
Fluorometric Intracellular Ros Kit, Cat. No. MAK145: Detection of ROS in Jurkat cells. Jurkat cells were seeded on the same day at 300,000 cells/100 μL/well in a black wall/clear bottom 96-well plate. The ROS assay loading solution (100 μL/well) was added and incubated in a 5% CO2, 37 °C incubator for 1 hour. The cells were treated with or without 1 mM H2O2 for 2 hours. The fluorescence signal was monitored at Ex/Em = 520/605 nm (cut off = 590 nm) with bottom read mode.
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| detection method | fluorometric |
| relevant disease(s) | neurological disorders; cancer |
| storage temp. | −20°C |
| usage | sufficient for 200 fluorometric tests (red) |
| Application: | Fluorometric intracellular reactive oxygen species (ROS) Kit has been used for detection of intracellular ROS in live cells. |
| Application: | This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope. |
| Biochem/physiol Actions: | The kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 520/λem = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader. |
| General description: | Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |