Fluorometric Sphingomyelin Assay Kit
SIGMA/MAK154 - sufficient for 100 fluorometric tests
Product Type: Chemical
Fluorometric Sphingomyelin Assay Kit, Cat. No. MAK154 : Sphingomyelin dose response was measured on a solid black 96-well plate. As low as 1 μM sphingomyelin can be detected with 60 minutes incubation (n=3).
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| detection method | fluorometric |
| relevant disease(s) | cancer |
| storage temp. | −20°C |
| usage | sufficient for 100 fluorometric tests |
| Application: | This kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts. |
| Biochem/physiol Actions: | Sphingomyelin is determined by a coupled enzyme assay, in which the hydrolysis of sphingomyelin by sphingomyelinase produces phosphocholine, which results in a fluorescent product (λex =540/λem = 590 nm) proportional to the Sphingomyelin present. |
| General description: | Sphinogmyelin, a sphingolipid consisting of a phospholipid (typically phosphocholine) attached to a ceramide moiety, is a major component of cellular membranes. Sphingomylein is enriched in the exoplasmic leaflet of the cell membrane. Within the cell membrane, sphingomyelin interacts with cholesterol to form lipid rafts. The metabolism of sphingomyelin contributes to cellular signaling via the release of ceramide, a second messenger that participates in multiple cellular signaling pathways. Sphingomyelin accumulates in Niemann-Pick disease due to deficiencies in sphingomyelinase activity. |
| Symbol |    GHS05,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H317 - H318 - H334 - H410 |
| Precautionary statements | P261 - P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-52/53 |
| Safety Statements | 61 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |