Renin Assay Kit
SIGMA/MAK157 - sufficient for 100 fluorometric tests
Synonym: Renin Enzyme Detection Kit
Product Type: Chemical
Renin Assay Kit, Cat. No. MAK157 : Renin dose response was measured in a 96-well black solid plate using a fluorescence microplate reader. As low as 10 ng/mL Renin was detected with 60 minutes incubation in 37 °C.
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| detection method | fluorometric |
| relevant disease(s) | cardiovascular diseases |
| storage temp. | −20°C |
| usage | sufficient for 100 fluorometric tests |
| Application: | Renin Assay Kit has been used to determine the activity of renin. |
| Application: | The Renin Assay Kit provides a convenient and simple method for screening renin activity in a variety of samples or for screening renin inhibitors. |
| Biochem/physiol Actions: | Renin activity is determined using a proprietary fluorescence resonance energy transfer (FRET) peptide containing a fluorescence quencher. Cleavage of the peptide by renin results in the production of a fluorescent product (λex = 540/λem = 590 nm). This assay is ~50-fold more sensitive than the EDANS/DABCYL-based assay. |
| General description: | Renin, also known as angiotensinogenase, is an aspartyl protease that cleaves angiotensinogen to form angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS). This system plays a critical role in the regulation of blood pressure and fluid balance. Increased renin activity can result in hypertension due to increased vasoconstriction and fluid retention. Inhibitors of renin can be used as a treatment for hypertension. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |