Mitochondrial Membrane Potential Kit
SIGMA/MAK160 - sufficient for 100 fluorometric tests (flow cytometry)
Synonym: JC-10 Assay
Product Type: Chemical
Mitochondrial Membrane Potential Kit, Cat. No. MAK160 : Effect of FCCP induced mitochondria membrane potential change in JurKat cells. JurKat cells were dye loaded with JC-10 dye-loading solution along with DMSO (Top) or 5 μM FCCP (Low) for 10 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-10 were measured with a flow cytometer using FL1 and FL2 channels. Uncompensated data (left column) were compared with compensated data (right column).
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| detection method | fluorometric |
| relevant disease(s) | cancer |
| storage temp. | −20°C |
| usage | sufficient for 100 fluorometric tests (flow cytometry) |
| Application: | Mitochondrial Membrane Potential Kit has been used to detect mitochondrial membrane potential (MMP) of PC-12 cells. |
| Application: | This kit is suitable for the flow cytometric detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators. |
| Biochem/physiol Actions: | This kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (λex = 540/λem = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (λex = 490/λem = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators. |
| General description: | Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |