Multidrug Resistance Assay Kit
SIGMA/MAK161 - (Fluorometric MDR Assay)
Synonym: MDR Assay
Product Type: Chemical
Multidrug Resistance Assay Kit, Cat. No. MAK 161 : Effect of Cyclosporin A on the inhibition of P-gp pump in MCF-7/ADR cells. The increased concentration of Cyclosporin A resulted in an increase in fluorescence signal caused by the inhibition of P-gp pump which enhanced the intracellular accumulation of MDR indicator dye. The EC50 = 2.4 μM is similar to the value reported in the literature.
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| application(s) | pharmaceutical |
| detection method | fluorometric |
| relevant disease(s) | cancer |
| storage temp. | −20°C |
| usage | (Fluorometric MDR Assay) |
| Application: | This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity. |
| Biochem/physiol Actions: | This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λEx = 490/λEm = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity. |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell. This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λex = 490/λem = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |