Intracellular Hydrogen Peroxide Assay
SIGMA/MAK164 - sufficient for 200 fluorometric tests (green fluorescence)
Synonym: Hydrogen Peroxide Detection in Cells
EC Number: 200-664-3
Product Type: Chemical
Intracellular Hydrogen Peroxide Assay, Cat. No. MAK164: Images of Live CHO-K1 cells in a black 96-well plate. Live CHO-K1 cells were stained. (A) Control cells. (B) Cells treated with 100 μM H2O2 at room temperature for 5 minutes.
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| detection method | fluorometric |
| relevant disease(s) | aging/geriatric diseases; neurological disorders; pulmonary disorders; orthopedic diseases; cardiovascular diseases |
| storage temp. | −20°C |
| usage | sufficient for 200 fluorometric tests (green fluorescence) |
| Application: | Intracellular hydrogen peroxide assay kit has been used to measure intracellular hydrogen peroxide levels. |
| Application: | This kit is suitable for the detection of hydrogen peroxide in living cells and a variety of samples such as cell extracts and liquids. |
| Biochem/physiol Actions: | The Intracellular Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in living cells and in a variety of other samples such as cellular extracts. This kit utilizes a unique cell-permeable sensor that generates a fluorescent product (λex = 490/λem = 520 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader, flow cytometer, or fluorescent microscope. |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |