Fluorimetric Hydrogen Peroxide Assay Kit
SIGMA/MAK165 - sufficient for 500 fluorometric tests (red fluorescence)
Synonym: Hydrogen Peroxide Quantification Kit
Product Type: Chemical
Fluorimetric Hydrogen Peroxide Assay Kit, Cat. No. MAK 165: H2O2 dose response was measured in a 384-well black plate using a fluorescence microplate reader. As low as 0.03 μM H2O2 can be detected with 30 minutes incubation (n=3). The insert shows the low levels of H2O2 detection.
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| detection method | fluorometric |
| relevant disease(s) | cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders |
| storage temp. | −20°C |
| usage | sufficient for 500 fluorometric tests (red fluorescence) |
| Application: | This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. |
| Biochem/physiol Actions: | The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λex = 540/λem = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader. |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |