Fluorimetric Hydrogen Peroxide Assay Kit
SIGMA/MAK166 - sufficient for 500 fluorometric tests (infrared fluorescence)
Synonym: Hydrogen Peroxide Detection Kit
EC Number: 231-765-0
Product Type: Chemical
Fluorimetric Hydrogen Peroxide Assay Kit, Cat. No. MAK 166 : H2O2 dose response was measured in a 96-well black plate. As low as 0.03 μM H2O2 can be detected with 1 minute incubation (n=3).
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| detection method | fluorometric |
| relevant disease(s) | orthopedic diseases; cardiovascular diseases; aging/geriatric diseases; neurological disorders; pulmonary disorders |
| storage temp. | −20°C |
| usage | sufficient for 500 fluorometric tests (infrared fluorescence) |
| Application: | Fluorimetric Hydrogen Peroxide Assay Kit has been sued to determine the oxidation pressure (H2O2). |
| Application: | The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. |
| Biochem/physiol Actions: | Fluorimetric Hydrogen Peroxide Assay Kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates an infrared fluorescent product (λex = 640/λem = 680 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader. The generation of the fluorescence product is pH independent between pH 4 to pH 10 and is superior to other fluorescence kits for measuring hydrogen peroxide in low pH solutions which often display decreased probe sensitivity. The infrared fluorescent probe is less sensitive to assay background due to autofluorescence from biological samples. This assay is compatible with high-throughput handling systems. |
| Features and Benefits: | This assay is compatible with high-throughput handling systems. |
| General description: | Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration. |
| Symbol |    GHS03,GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H271 - H302 + H332 - H314 |
| Precautionary statements | P220 - P280 - P305 + P351 + P338 - P310 |
| Hazard Codes | O,C |
| Risk Statements | 5-8-20/22-35 |
| Safety Statements | 17-26-28-36/37/39-45 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |