High Sensitivity Pyrophosphate Assay Kit
SIGMA/MAK169 - sufficient for 200 fluorometric tests
Synonym: Pyrophosphate (PPi) Assay Kit
EC Number: 200-664-3
Product Type: Chemical
High Sensitivity Pyrophosphate Assay Kit, Cat. No. MAK169 : Pyrophosphate dose responses was measured in a solid black 96-well plate. As low as 1 μM (100 picmoles/well) pyrophosphate can be detected with 10 minutes incubation.
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| application(s) | cosmetics food and beverages |
| detection method | fluorometric |
| relevant disease(s) | genitourinary disorders; rheumatological diseases |
| storage temp. | −20°C |
| usage | sufficient for 200 fluorometric tests |
| Application: | The Pyrophosphate Assay Kit provides a simple and direct flourescent microplate procedure for measuring pyrophosphate in a variety of samples. |
| Biochem/physiol Actions: | The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =370/λem = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors. |
| General description: | Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |