Universal Fluorometric Kinase Assay Kit
SIGMA/MAK173 - sufficient for 250 fluorometric tests
Synonym: Universal Kinase Test Kit
EC Number: 200-664-3
Product Type: Chemical
Universal Fluorometric Kinase Assay Kit, Cat. No. MAK173 : ADP dose response was measured in a solid black 384-well plate. As low as 0.3 μM ADP can be detected with 30 minutes incubation (Z’ factor =0.65).
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| detection method | fluorometric |
| relevant disease(s) | endocrinological disorders, diabetes; neurological disorders; cardiovascular diseases; cancer |
| storage temp. | −20°C |
| usage | sufficient for 250 fluorometric tests |
| Application: | Suitable for the determination of kinase activity and for screening and identifying kinase inhibitors |
| Biochem/physiol Actions: | This Universal Fluorimetric Kinase Assay Kit is based on monitoring ADP formation, which is directly proportional to kinase activity. This enzyme-coupled kit provides a fast, efficient, and homogeneous assay to measure kinase activities. This assay kit is a non-radioactive and non-antibody based method to detect the amount of ADP produced from enzyme reaction without a separation step. This kit demonstrates high sensitivity (<0.3 μM ADP) and broad ATP tolerance (1-300 μM. This kit can be utilized for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. |
| General description: | Protein kinases are the enzymes that catalyze the transfer of phosphate group from a phosphate donor to an acceptor in a substrate protein. Kinases are of great interest to researchers due to their role in cell signaling and their roles in disease states. Most of the commercial protein kinase assay kits are based on monitoring either phosphopeptide formation or the ATP depletion. Kinase assays that rely on the detection of phosphopeptides require significant effort to identify an optimized peptide for the specific kinase of interest. The ATP depletion method suffers various interferences due to inhibition or activation of luciferase by compounds found in the biological matrix. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P264 - P270 - P301 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |