Citrate Synthase Activity Assay Kit
SIGMA/MAK193 - Sufficient for 100 Colorimetric tests
Synonym: Citrate Synthase Test Kit
EC Number: 232-821-7
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
Chart Citrate Synthase Activity Assay Kit: Cat. No. MAK193: (a) GSH Standard Curve, (b) CitRate Synthase Activity in Jurkat cell lysate (10μg), Rat liver lysate (20 μg), purified yeast mitochondria (4 μg) and CS Positive Control (2 μL).
| detection method | colorimetric |
| relevant disease(s) | endocrinological disorders, diabetes; cancer |
| storage temp. | −20°C |
| Application: | Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples. |
| Application: | Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria. |
| Biochem/physiol Actions: | The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2. |
| General description: | Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |
| Components | CS Assay Buffer; CS Substrate Mix; CS Developer; GSH Standard, Reduced; CS Positive Control |