Enrofloxacin Extraction Kit
SIGMA/MAK226 - Sufficient for 40 extractions
Product Type: Chemical
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12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| application(s) | cosmetics food and beverages sample preservation |
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Waste Prevention Learn more about the Principles of Green Chemistry . |
| storage temp. | room temp |
| sustainability | Greener Alternative Product |
| Application: | Enrofloxacin concentrations (ng/g) can be derived from biological specimens. Can be used for any biological food sample-shrimp, meat, liver, crab fish, etc. This kit enables the extraction of enrofloxacin for quantitation by ELISA. Click on the following link for a video demonstration of how the kit works |
| Application: | This kit is suitable for the extraction of enroflaxacin from a variety of samples such as biological tissue and food samples. |
| Biochem/physiol Actions: | The Enrofloxacin Extraction Kit enables the simultaneous separation of enrofloxacin from tissue, and the defatting of the sample during extraction in an accurate and quick manner. The streamlined process bypasses the centrifugation, vortexing, pipetting and solvent addition steps associated with extracting and defatting the sample. The extracted enrofloxacin can then be detected and quantified by enzyme-linked immunosorbent assay (ELISA) followed by spectrophotometric analysis. Enrofloxacin is typically extracted using a single solvent system containing 80% methanol in buffer. In the standard procedure, methanol containing the enrofloxacin is separated from tissue by centrifugation for 10 minutes followed by manual pipetting. The extract is then dried and reconstituted into 1 mL of 8% methanol in sample dilution buffer. The extract is subsequently defatted by adding 1 mL hexane and centrifuging. A 50 μL aliquot is taken from the bottom layer containing the defatted enrofloxacin extract and used for analysis by ELISA and spectrophotometry. The Enrofloxacin Extraction Kit shortcuts the extraction process by eliminating the need for centrifugation, manual pipetting, and post-extraction defatting with hexane or another lipophilic solvent. After adding 80% methanol to the sample, it can be mixed manually for 15 minutes or with sonication for 5 minutes and poured through the syringe containing a filter. The eluted solution containing the enrofloxacin extract is then reconstituted in 8% methanol and a 50 μL aliquot is used for analysis by ELISA and spectrophotometry. |
| Features and Benefits: | The streamlined process bypasses the centrifugation, vortexing, pipetting and solvent addition steps associated with extracting and defatting the sample. The extracted enrofloxacin can then be detected and quantified by enzyme-linked immunosorbent assay (ELISA) followed by spectrophotometric analysis. |
| General description: | Enrofloxacin is a fluoroquinolone antibiotic used for the treatment of pets and domestic animals and in fish farming. Minimum acceptable limits have been set for enrofloxacin residues in food samples. The enrofloxacin extraction kit enables the simultaneous separation of enrofloxacin from tissue, and the defatting of the sample during extraction in an accurate and quick manner. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here . |
| Symbol |    GHS02,GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H225 - H301 + H311 + H331 - H370 |
| Precautionary statements | P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 2 |
| Flash Point(F) | 49.5 °F |
| Flash Point(C) | 9.7 °C |
| Storage Temp. | room temp |
| UNSPSC | 12161503 |