Thrombin Activity Fluorometric Assay Kit
SIGMA/MAK242 - sufficient for 100 fluorometric tests
Synonym: Thrombin Enzyme Activity Kit
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| detection method | fluorometric |
| relevant disease(s) | hematological disorder |
| storage temp. | −20°C |
| Application: | Suitable for determining the activity of pure Thrombin and for detecting the activity of Thrombin in plasma. |
| Biochem/physiol Actions: | Thrombin activity fluorometric assay kit utilizes the ability of thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, 7-amino-4-methylcoumarin (AMC), which can be easily quantified by fluorescence. This assay kit is simple, rapid and can detect thrombin activity as low as 1ng in samples. |
| General description: | Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |