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GAPDH Activity Assay Kit

SIGMA/MAK277 - sufficient for 100 colorimetric tests

Synonym: Glyceraldehyde-3-phosphate Dehydrogenase Activity Assay Kit

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-MAK277-1KT 1 kit
 DISCONTINUED  
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detection method colorimetric
relevant disease(s) cancer
storage temp. −20°C
Application: GAPDH Activity Assay Kit has been used to measure the enzyme activity of glyceraldehyde-3-phosphate dehydrogenase in testis.
Application: Suitable for use with various tissue and cell culture samples.
Biochem/physiol Actions: The GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. GAPDH activity is determined in a coupled enzyme reaction in which GAP is converted to BPG by GAPDH. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. The assay is sensitive to 100 mUnits/mL. One unit of GAPDH activity is the amount of enzyme that will generate 1.0 mmole of NADH per minute at pH 7.2 at 37 °C.
Features and Benefits: Compatible with high-throughput handling systems.
General description: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.
Storage Temp. −20°C
UNSPSC 12161503

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