Bile Acid Assay Kit
SIGMA/MAK309 - sufficient for 100 fluorometric tests
Synonym: Biliary Acid Quantification Kit; Total Bile Acids Assay Kit
Product Type: Chemical
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| detection method | fluorometric |
| relevant disease(s) | gastrointestinal diseases; cancer; cardiovascular diseases |
| storage temp. | −20°C |
| Application: | Bile Acid Assay Kit has been used to quantify bile acid levels from plasma. |
| Application: | Suitable for the quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism in serum, plasma, urine and other biological samples. |
| Biochem/physiol Actions: | This Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3–hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (γex = 530 nm/γem = 585 nm) is linear to the bile acid concentration in the sample. |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gall stones, colon cancer, etc. Bile acids also exist as sulfate salt forms known as bile acid sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |