Peroxide Assay Kit
SIGMA/MAK311 - sufficient for 250 colorimetric tests
Synonym: Hydrogen Peroxide Test Kit
Product Type: Chemical
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| detection method | colorimetric |
| relevant disease(s) | cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders |
| storage temp. | 2-8°C |
| Application: | Peroxide Assay Kit has been used to measure hydrogen peroxide level in rat tissue homogenates. |
| Application: | Suitable for the quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. |
| Biochem/physiol Actions: | The Peroxide Assay Kit provides a simple and high-throughput adaptable assay for quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. This assay utilizes the chromogenic Fe3+ −xylenol orange reaction, in which a purple complex is formed when Fe2+ is oxidized to Fe3+ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present. The optimized formulation reduces interference by substances in the raw samples. A concentration of 1 μM H2O2 equals 34 ng/mL or 34 ppb. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H2O2 in a 96 well format. |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Peroxide (e.g. hydrogen peroxide H2O2), a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 - H315 - H319 |
| Precautionary statements | P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 |
| RIDADR | UN 2796 8 / PGII |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161503 |