Heme Assay Kit
SIGMA/MAK316 - sufficient for 250 colorimetric tests
Synonym: Heme Quantification Kit
Product Type: Chemical
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| detection method | colorimetric |
| relevant disease(s) | hematological disorder |
| storage temp. | 2-8°C |
| Application: | Suitable for the quantitative determination of heme and evaluation of drug effects on heme metabolism in a variety of biological samples such as blood, plasma, serum, urine, and heme carrying enzymes. |
| Biochem/physiol Actions: | Heme assay is based on an aqueous alkaline solution method, in which the heme is converted into a uniform colored form, producing a colorimetric (400 nm) result, directly proportional to the heme concentration in the sample. The optimized formulation reduces interference and exhibits high sensitivity. One mg/dL heme equals 15.3M, 0.001%, or 10ppm. The kit has a linear detection range of 0.6–125M heme in a 96 well format. |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm, and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalases and peroxidases. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases. |
| Symbol |   GHS05,GHS09 |
| Signal word | Warning |
| Hazard statements | H290 - H319 - H410 |
| Precautionary statements | P234 - P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161503 |