Sorbitol Dehydrogenase Assay Kit
SIGMA/MAK317 - sufficient for 100 colorimetric tests
Product Type: Chemical
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| detection method | colorimetric |
| relevant disease(s) | endocrinological disorders, diabetes; gastrointestinal diseases |
| storage temp. | −20°C |
| Application: | Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media. |
| Biochem/physiol Actions: | Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media). |
| Features and Benefits: | Compatible with high-throughput handling systems. |
| General description: | Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy. |
| Symbol |    GHS02,GHS05,GHS07 |
| Signal word | Warning |
| Hazard statements | H226 - H290 - H315 - H319 - H336 |
| Precautionary statements | P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338 |
| RIDADR | UN 1993C 3 / PGIII |
| Flash Point(F) | 75.2 °F - closed cup |
| Flash Point(C) | 24 °C - closed cup |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |