Mammalian Cell Lysis Kit
SIGMA/MCL1
Synonym: Cell Lysis Reagent
Product Type: Chemical
Whole cell protein extract prepared from PC-12 and HeLa cell lines using Sigma′s mammalian cell lysis kit (S) or competitor (C). The arrows indicate the MAPK protein products. The numbers indicate the protein size markers in kDa.
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| usage | kit sufficient for 250 extractions (100 mm tissue culture plates) |
| Application: | The product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays. Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration. |
| Features and Benefits: | • The kit can be used to prepare cell lysis buffer and other modified buffers • The kit enables the researcher to change the buffer′s components to obtain maximum efficiency for a specific protein |
| Other Notes: | The mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions. |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H412 |
| Precautionary statements | P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 21/22-36/37/38 |
| Safety Statements | 26-36/37 |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | Protease inhibitor cocktail 2.5 mL |