Imprint® DNA Modification Kit
SIGMA/MOD50 - For bisulfite DNA conversion & purification
Synonym: Methylation-Specifc PCR preparation; bisulfite conversion kit; bisulphite modification
EC Number: 231-548-0
Product Type: Chemical
Superior Sensitivity. Bisulfite modification was performed on decreasing concentrations of human genomic DNA using Imprint (Cat. No. MOD50) and kits from two other suppliers. Methylation-specific PCR (MSP) was then performed on all samples using β-actin gene-specific primers (109 bp amplicon). For all suppliers lanes from left to right are 100 ng, 10 ng, 1 ng, 100 pg, 50 pg and no sample, respectively. Suppliers C and D (not shown) have no product at 50 pg. These results demonstrate the superior sensitivity of the Imprint kit relative to other suppliers.
Best-in-Class Yield and Conversion (MOD50). 100 ng of human genomic DNA was bisulfite treated according to each supplier’s protocol followed by qPCR analysis. The modified and unmodified primer sets within the β-actin gene were then used to determine bisulfite yield. Ct values were compared to the Ct value of a 10-ng human genomic standard. Percent conversion was determined after busulfite modification of 100 ng of human genomic DNA. Comparison of qPCR delta Ct values between modified and unmodified primers within β-actin demonstrates conversion rates. The graph on the left highlights the superior yield and conversion rates of the Imprint kit in relation to other suppliers.
| Quality Level | 200  |
| storage temp. | 20-25°C |
| usage | sufficient for 50 reactions |
| Application: | Imprint® DNA Modification Kit has been used for bisulfite modification of DNA. |
| Features and Benefits: | • Only 50 picograms of DNA or 20 cells are required • Procedure takes less than 2 hours • Greater than 99% conversion rate • Extremely low degradation • Option of convenient one-step protocol • Consistent and reproducible Bisulfite Modification • Can be used with genomic, endonuclease digested, and FFPE DNA |
| General description: | The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray. |
| Legal Information: | Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes. |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H290 - H302 - H314 - H412 |
| Precautionary statements | P234 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-31 |
| Safety Statements | 25-46 |
| RIDADR | UN 3316 9 |
| Supplemental Hazard Statements | EUH031 |
| Storage Temp. | 20-25°C |
| UNSPSC | 41116158 |
| Components | Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural |