Sanger Arrayed Whole Genome Lentiviral CRISPR Library
SIGMA/MSANGERG - Mouse, Glycerol Format
Synonym: CRISPR library; Sanger CRISPR library
Product Type: Chemical
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| application(s) | CRISPR |
| packaging | pkg of 50 μL (96-well plate) |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Functional Genomics/Screening /Target Validation |
| Application: | Sanger Arrayed Whole Genome Lentiviral CRISPR Library has been used for Functional Genomics/Screening /Target Validation. |
| Features and Benefits: | • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only) • Simplify the workflow with puromycin selection • Illuminate CRISPR-expressing cells with BFP • Flip the expression components into and out of the genome using transposase Additional Features • Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments • Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells • Collaborative: Real-time, library validation continues For detailed information on the Sanger library, click here |
| General description: | Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery. |
| Legal Information: | CRISPR Use License Agreement Lentiviral License Agreement |
| Other Notes: | Additional libraries are available: Human, Glycerol Format Human, Virus Format Mouse, Virus Format |
| Other Notes: | Bacteria glycerol stock of gRNA-only 3rd generation lenti-based plasmid vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones Request a Quote or More Information |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination. |
| Packaging: | 96-well plates |
| Physical form: | 50 μl of bacterial glycerol stocks, 450×96-well plates |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41105904 |