Sanger Arrayed Whole Genome Lentiviral CRISPR Library
SIGMA/MSANGERV - Mouse, Virus Format
Synonym: Arrayed CRISPR library; Sanger CRISPR library
Product Type: Chemical
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| application(s) | CRISPR |
| concentration | 1x106 VP/ml (via p24 assay) |
| packaging | pkg of 10 μL (384-well plate) |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Functional Genomics/Screening /Target Validation |
| Features and Benefits: | • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only) • Simplify the workflow with puromycin selection • Illuminate CRISPR-expressing cells with BFP Additional Features • Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening • Collaborative: Real-time, library validation continues For detailed information on the Sanger library, click here |
| General description: | Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery. |
| Legal Information: | CRISPR Use License Agreement Lentiviral License Agreement |
| Other Notes: | Additional libraries are available: Human, Glycerol Format Mouse, Glycerol Format Human, Virus Format |
| Other Notes: | Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones Request a Quote or More Information |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination. |
| Packaging: | 384 well plates |
| Physical form: | 10μl of lentivirus at ≥106 particles/ml in ~120×384 well plates |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41105904 |