Membrane Scaffold Protein 1D1(-)
SIGMA/MSP03 - recombinant, expressed in E. coli, buffered aqueous solution
Synonym: Membrane Scaffold Protein
Product Type: Chemical
Protein Maps of Apolipoprotein A-1 and MSP1D1(-). The original MSP1 sequence omits the Globular Region N-terminal domain of native Apo A-1. MSP1D1(-) omits both the histidine-tag and the TEV protease-recognition sequence in MSP1D1.
Fifteen different Membrane Scaffold Protein Constructs are available for applications that require tagging, chemical modifications and acommodations for various size membrane proteins.
| assay | ≥90% (SDS-GE) |
| form | buffered aqueous solution |
| mol wt | 22,043.9 Da |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | ambient |
| solubility | water: soluble |
| storage temp. | −20°C |
| Application: | For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page . |
| Biochem/physiol Actions: | Nano-disctechnology is an approach that allows aqueous soluble membrane proteins in anative-like bilayer environment that enables the membrane proteins to remainactive and stable. The concept of this technology is derived from high-densitylipoprotein (HDL) particles and apolipoprotein, their primary proteincomponent. The nano-disc is a non-covalent structure consisting of MSP that isgenetically engineered to mimic the activity of apolipoprotein A1 (Apo-A1) andphospholipid bilayer. In the presence of synthetic phospholipids, MSP1D1 canself-assemble into discoidal nanoparticlesknown as nano-discs. These nano-discs are suitable for studying membraneproteins and are applicable in the field of medicine and biotechnology. |
| General description: | Research area: Cell structure. Membrane Scaffold Protein 1D1 (MSP1D1) is a variant of MSP1 where the first eleven amino acids in the helix 1 portion are deleted of the original MSP1 sequence. It consists of an N-terminal histidine-tagged protein with a tobacco etch virus (TEV) protease cleavage site that is present between the protein sequence and histidine-tag. MSP1D1(–)variant of MSP1D1 lacks the histidine-tag and the TEV protease-recognition sequence. |
| Legal Information: | Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois. • 7,691,414 Membrane scaffold proteins • 7,662,410 Membrane scaffold proteins and embedded membrane proteins • 7,622,437 Tissue factor compositions and methods • 7,592,008 Membrane scaffold proteins • 7,575,763 Membrane scaffold proteins and tethered membrane proteins • 7,083,958 Membrane scaffold proteins • 7,048,949 Membrane scaffold proteins |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-GE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |