Membrane Scaffold Protein 1E3D1
SIGMA/MSP08 - recombinant, expressed in E. coli, buffered aqueous solution
Synonym: Membrane Scaffold Protein 1E3D1; MSP1E3D1
Product Type: Chemical
The concept of membrane scaffold proteins was originally derived from the naturally occurring apoliporotein A-I component of high density lipoprotein. Phospholipid bilayer nanodiscs have been utilized to incorporate a wide variety of anchored and transmembrane proteins. Nanodiscs can provide improved solubility, homogeneity and more native conditions over traditional microsomal preparation.
The critical component of Nanodiscs is the encircling amphipathic helical protein belt (membrane scaffold protein or MSP). The MSP1D1 construct generates Nanodiscs ~9.8 nm in diameter typically containing ~150 lipid molecules per Nanodisc. The thickness of a Nanodisc can vary depending on the type of phospholipid incorporated (typically 4.6–5.6 nm).
Our two constructs, MSP1D1 and MSP1E3D1, allow the incorporation of a broad range of target protein sizes, generating Nanodiscs of ~10.6 and ~12.9 nm in diameter, respectively.
Protein Maps of Apolipoprotein A-1 and MSP1E3D1. The original MSP1 sequence omits the Globular Region N-terminal domain of native Apo A-1. MSP1D1 deletes the first 11 amino acids in the Helix 1 portion (H0.5 in the figure) of the original MSP1 sequence. MSP1E3D1 repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.
Fifteen different Membrane Scaffold Protein Constructs are available for applications that require tagging, chemical modifications and acommodations for various size membrane proteins.
| assay | ≥90% (SDS-GE) |
| form | buffered aqueous solution |
| mol wt | 32,599.6 Da |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | ambient |
| solubility | water: soluble |
| storage temp. | −20°C |
| Application: | For an extensive list of citations and protocols visit the Sligar Lab Website at; sligarlab.life.uiuc.edu/n |
| Application: | For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page . |
| Application: | Nanodisc soluble lipid bilayer systems have proven to be a widely applicable means for rendering membrane proteins soluble in aqueous solutions in a native-like bilayer environment where they remain monodisperse and active. The critical component of nanodiscs is the encircling amphipathic helical protein belt (membrane scaffold protein). |
| Application: | The nanodisc system has been employed to incorporate a wide variety of proteins including GPCRs, P450s, bacteriorhodopsin, coagulation factors, cholera toxin, TAR receptor and aromatase. |
| Biochem/physiol Actions: | Generates Nanodiscs ~12.9 nm in diameter |
| General description: | Research area: Cell Struc Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 variant of MSP1 differs from MSP1 in the following facets: It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence3 (which is known separately as MSP1D1). It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1. |
| Legal Information: | Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois. • 7,691,414 Membrane scaffold proteins • 7,662,410 Membrane scaffold proteins and embedded membrane proteins • 7,622,437 Tissue factor compositions and methods • 7,592,008 Membrane scaffold proteins • 7,575,763 Membrane scaffold proteins and tethered membrane proteins • 7,083,958 Membrane scaffold proteins • 7,048,949 Membrane scaffold proteins |
| Legal Information: | Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois. • 7,691,414 Membrane scaffold proteins • 7,662,410 Membrane scaffold proteins and embedded membrane proteins • 7,622,437 Tissue factor compositions and methods • 7,592,008 Membrane scaffold proteins • 7,575,763 Membrane scaffold proteins and tethered membrane proteins • 7,083,958 Membrane scaffold proteins • 7,048,949 Membrane scaffold proteins |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-GE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |