SILu™MAb Mouse Stable-Isotope Labeled Monoclonal Antibody
SIGMA/MSQC10 - from mouse, recombinant, expressed in CHO cells
Product Type: Chemical
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| antibody product type | primary antibodies |
| assay | ≥90% (SDS-PAGE) |
| biological source | mouse |
| packaging | vial of 100 μg |
| Quality Level | 200  |
| recombinant | expressed in CHO cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| General description: | SILu™MAb Mouse is a recombinant, stable isotope-labeled, mouse monoclonal antibody which incorporates [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in CHO cells, SILu™MAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal murine antibodies in animal pharmacokinetics (PK) studies. SILu™MAb Mouse is an IgG1 antibody with a kappa light chain. Because of overlap with common sequences in the Fc region with candidate murine antibodies, SILu™MAb Mouse provides universal utility, and thus, eliminates the need to produce candidate-specific internal standards. |
| Legal Information: | SILu is a trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Each vial of SILu™MAb Mouse contains the labeled antibody lyophilized from a solution of phosphate buffered saline. Vial content was determined by measuring A280 and using an extinction coefficient (E0.1%) of 1.4. |
| Preparation Note: | Produced utilizing enriched media containing stable isotope labeled amino acids such as [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in CHO cells, SILuMAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal murine antibodies in animal pharmacokinetics (PK) studies |
| Preparation Note: | SILu™MAb Mouse recovery is maximized when 0.1% formic acid is used for reconstitution of the lyophilized product. Reconstitution with other solvents may reduce recovery. Do not freeze after reconstitution. • Briefly centrifuge the vial at ~10,000 × g to collect the product at the bottom of the vial. • Add 500 μL of ultrapure water containing 0.1% formic acid to the vial. • Mix the contents by gently inverting the vial a minimum of 5 times. • Allow the vial to stand at room temperature for at least 15 minutes and repeat mixing by inversion. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |