MS-SAFE Protease and Phosphatase Inhibitor
SIGMA/MSSAFE
Synonym: Mass Spectrometry Safe Protease and Phosphatase Inhibitor Cocktail
Product Type: Chemical
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| description | for use with mammalian cell and tissue extract, lyophilized powder |
| form | powder |
| Quality Level | 200  |
| solubility | water: 0.1 g/mL, clear, colorless |
| storage temp. | 2-8°C |
| Application: | MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity. |
| Application: | Tested in mammalian cell lysates and liver tissue extracts. Designed for use in samples to be analyzed by mass spectrometry. |
| Biochem/physiol Actions: | Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases |
| Features and Benefits: | Comprehensive protease and phosphatase inhibitor cocktail for mass spectrometry analysis Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment Allows accurate measurement of protein activity and identification of phosphorylation sites. |
| General description: | Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of: • Serine, cysteine and aspartic proteases, and metalloproteases • Tyrosine, serine/threonine, acid and alkaline phosphatases All inhibitors and fillers were chosen so as not to interfere with LC-MS analyses. Any inhibitors capable of covalent, irreversible protein modification were avoided. This cocktail is EDTA-free. All inhibitors were selected to allow facile downstream sample processing, such as immobilized metal affinity chromatography (IMAC) for His-tagged protein purification and, uniquely, phosphopeptide enrichment. |
| Other Notes: | Protease inhibitors: • Bestatin hydrochloride • Leupeptin • Phosphoramidon disodium salt • Pepstatin A • Elastatinal • Aprotinin • Nafamostat mesylate • Antipain Phosphatase inhibitors • Okadaic acid • Sodium fluoride • Sodium orthovanadate • Bromotetramisole oxalate Fillers • β-lactose • DL-leucine |
| Packaging: | 1 vial in glass bottle |
| Physical form: | Lyophilized powder that is water-soluble |
| Preparation Note: | One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P301 + P312 + P330 |
| Hazard Codes | Xn |
| Risk Statements | 22-32 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |