SILu^™Lite APOA1 Apolipoprotein A-1 human

SIGMA/MSST0002 - recombinant, expressed in HEK 293 cells, MS Protein Standard

Synonym: SILuLite Apolipoprotein A-1

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-MSST0002-50UG	50 µg	$836.00 1/EA	Add To Favorites

SILuProt products are stable isotope-labeled full length proteins designed to be used as mass spectrometry internal standards for quantitative proteomics. Introduction of an internal SILuProt standard at the beginning of the MS workflow minimizes variations in quantification due to protein extraction, fractionation, enrichment, proteolysis and analysis

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (SDS-PAGE)
biological source	human
form	lyophilized powder
packaging	vial of 50-65 μg (Lot-specific vial content given on certificate of analysis)
Quality Level	200
recombinant	expressed in HEK 293 cells
storage temp.	−20°C
tag	His tagged
	V5 tagged
technique(s)	mass spectrometry (MS): suitable
UniProt accession no.	P02647

Analysis Note:	General Description SILuLite APOA1 is a recombinant human protein expressed in human 293 cells. It is a monomer of 286 amino acids (including N-terminal signal sequence and C-terminal polyhistidine and V5 tags), with a molecular weight of 32.1 kDa. SILuLite APOA1 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications. Sequence RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQSDPSRGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ The N-terminal signal peptide and C-terminal linker peptide, V5 and polyhistidine tags are italicized. Other Characterization • Sequence confirmed by intact mass analysis • Identity verified by peptide mapping • purity >98% (SDS-PAGE) • Vial content was determined by the Bradford method using BSA as a calibrator. The correction factor of Bradford-to-AAA is 88.33% Suggested Quantitative Analysis Parameters (MRM settings provided for three suggested peptides )
Biochem/physiol Actions:	Apolipoprotein A-1(Apo-A1), is a major protein component of high density lipoprotein (HDL)in plasma¹. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters². Apo-A1 was shown to assist in the improvement of predicting cardiovascular events³. The gene that encodes to Apolipoprotein A-1 is APOA1. This gene is closely linked with two other apolipoprotein genes on chromosome 11⁴. Defects in this gene are associated with HDL deficiencies, including Tangier disease⁵, and with systemic non-neuropathic amyloidosis⁶. The protein is made up of one major isoform (pI 5.6) and two minor isoforms (pI 5.53 and 5.46). Apo-A1 shows a high content of a-helix structure⁶. The amphipathic regions in the α-helix structure seem to be responsible for lipid binding capacity. In aqueous solution, Apo-A1 shows self-association with minor conformational change⁷. In addition, it has been shown that Apo-A1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin⁸.
Legal Information:	SILu is a trademark of Sigma-Aldrich Co. LLC
Physical form:	Supplied as a lyophilized powder containing phosphate buffered saline.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (SDS-PAGE)
Storage Temp.	−20°C
UNSPSC	23201100

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