SILu™Lite VEGFA Vascular endothelial growth factor A human
SIGMA/MSST0006 - recombinant, expressed in HEK 293 cells, MS Protein Standard
Synonym: SILuLite Vascular endothelial growth factor A
Product Type: Chemical
SILuProt products are stable isotope-labeled full length proteins designed to be used as mass spectrometry internal standards for quantitative proteomics. Introduction of an internal SILuProt standard at the beginning of the MS workflow minimizes variations in quantification due to protein extraction, fractionation, enrichment, proteolysis and analysis
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | mass spectrometry (MS): suitable |
| UniProt accession no. | P15692  |
| Biochem/physiol Actions: | VEGF165 belongs to the PDGF/VEGF growth factor family characterized by the presence of eight conserved cysteine residues and a cystine knot structure.1 VEGF is secreted by the majority of tumor cells and initiates angiogenesis by activating endothelial cells of existing blood vessels and promoting their migration.2 VEGF has also been implicated in correlation with poor prognosis in breast cancer.2 In addition, VEGF is released in rheumatoid arthritis in response to TNF-α, increasing endothelial permeability and stimulating angiogenesis (formation of capillaries)3,4 |
| General description: | SILu™ Lite VEGF165 is a recombinant glycosylated human protein expressed in human 293 cells. It is a homodimer with an apparent molecular mass of 45 kDa. Suggested Quantitative Analysis Parameters (MRM settings provided for three suggested peptides ) |
| Legal Information: | SILu is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | APMAEGGGQNHHEVVKFMDVYQRSY |
| Physical form: | Supplied as a lyophilized powder containing phosphate buffered saline |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 23201100 |