SILu™Prot ALB Albumin human
SIGMA/MSST0011 - recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
Synonym: Albumin; LC/MS internal standard for human albumin in; stable isotope-labeled human albumin
Product Type: Chemical
SILuProt products are stable isotope-labeled full length proteins designed to be used as mass spectrometry internal standards for quantitative proteomics. Introduction of an internal SILuProt standard at the beginning of the MS workflow minimizes variations in quantification due to protein extraction, fractionation, enrichment, proteolysis and analysis
Human serum albumin is a ~69 kDa predominantly monomeric protein with one free sulfhydryl group and 17 intrachain disulfide bonds.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| concentration | ≥10 μg/mg (Bradford) |
| form | lyophilized powder |
| potency | ≥98% Heavy amino acids incorporation efficiency by MS |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| suitability | suitable for mass spectrometry (standard) |
| tag | His tagged |
| technique(s) | mass spectrometry (MS): suitable |
| UniProt accession no. | P02768  |
| Biochem/physiol Actions: | Albumin is synthesized exclusively by the liver parenchymal cells at a rate of 14 g/day. While it is the most abundant plasma protein (>50%), increased urine albumin concentrations indicate bleedings in the lower urinary tract (ureter, bladder) or infections of the renal pelvis. A small abnormal albumin excretion is known as Microalbuminuria and serves as an indicator for temporary overload of the glomerular filtration (fever, excessive sports) or chronic injury of the glomeruli (diabetes). |
| General description: | SILu™Prot ALB is a recombinant, stable isotope-labeled human albumin which incorporates [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in human 293 cells, it is designed to be used as an internal standard for bioanalysis of albumin in mass-spectrometry. SILu™Prot ALB is a monomer of 607 amino acids (including C-terminal polyhistidine and flag tags), with a molecular weight of ~69 kDa. |
| Legal Information: | SILu is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | RRDAHKSEVAHRFKDLGEENFKALV |
| Physical form: | Supplied as a lyophilized powder containing phosphate buffered saline. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 23201100 |