SILu™Lite APOB48, Apolipoprotein B-48 human
SIGMA/MSST0056 - recombinant, expressed in HEK 293 cells, MS Protein Standard
Synonym: APOB48; Apo B-48; Mass spectrometry standard, Apo B-48; Mass spectrometry standard, Apolipoprotein B-48
Product Type: Chemical
SILuProt products are stable isotope-labeled full length proteins designed to be used as mass spectrometry internal standards for quantitative proteomics. Introduction of an internal SILuProt standard at the beginning of the MS workflow minimizes variations in quantification due to protein extraction, fractionation, enrichment, proteolysis and analysis
| assay | ≥95% (SDS-PAGE) |
| form | liquid |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| shipped in | dry ice |
| storage temp. | −20°C |
| suitability | suitable for mass spectrometry (internal calibrator) |
| tag | FLAG® tagged |
| His tagged | |
| UniProt accession no. | P04114  |
| Biochem/physiol Actions: | Apolipoprotein B 48 acts as a ligand for low-density lipoprotein (LDL)-receptor. Elevated levels of APOB is associated with the development of elevated LDL levels and other lipid metabolic disorders. In addition, overexpression of the gene increases the risk of susceptibility to coronary heart disease. |
| Biochem/physiol Actions: | Apolipoprotein B-48 (Apo B-48) is known to be the only specific marker of intestinal chylomicron (CM) particles. The amino acid sequence of apoB-48 represents 48% of the initial sequence of apolipoprotein B-100 (Apo B-100). Apo B-48 is synthesized only by the intestine in humans, while Apo B-100 is synthesized primarily by the liver. Therefore, Apo B-48 is a most appropriate biomarker for cardiovascular and nutritional investigation of postprandial CM metabolism.In a recent study, Apo B-48 was discovered as a novel biomarker in amyotrophic lateral sclerosis patient fibroblasts. |
| General description: | Apolipoprotein B 48 (APOB48) is a key subunit of APOB. APOB is encoded by the gene mapped to human chromosome 2p24.1. |
| General description: | SILu™Lite APOB48 is a recombinant human protein expressed in human 293 cells. It consists of 2172 amino acids, with a calculated molecular mass of 243.2 kDa (including C-terminal polyhistidine and flag tags). SILu™Lite APOB48 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications. |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | SILu is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | EEEMLENVSLVCPKDATRFKHLRKY |
| Physical form: | Supplied as a liquid containing phosphate buffered saline. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 23201100 |