HepaRG™ 5F Control Cells
SIGMA/MTOX1010P24 - one assay ready, 24 well plate
Product Type: Chemical
HepaRG 5F Cells: 10× Magnification
hCAR KO HepaRG Data : Real-time PCR validation of mRNA expression of CYP2B6. Wild type and hCAR-KO HepaRG cells were cultured and treated with vehicle control (0.1% DMSO), Ritonavir, Phenobarbitol (PB), Rifampicin, CITCO, CBZ, Phenytoin and Dex, and mRNA expression of CYP2B6 was measured. CITCO, a selective hCAR agonist, did not induce CYP2B6 in hCAR KO HepaRG cells.
| biological source | human female liver (Source Disease: Hepatocarcinoma and Hepatitis C) |
| form | liquid |
| Quality Level | 200  |
| storage temp. | room temp |
| Analysis Note: | Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation |
| Application: | See technical bulletin for detailed protocols |
| Features and Benefits: | Sigma’s HepaRG 5F Clone exhibits improved growth characteristics and enhanced functionality, expressing a large panel of drug metabolism enzymes; including cytochrome P450 enzymes CYP1A2, CYP2B6, CYP2C8/9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4, nuclear receptors AhR, PXR, CAR, LXR, and FXR, and phase II enzymes UGT, SULT, NAT and GST. • The 24 well format provides delivery of ready-to-use liver cells for performing various experiments. |
| General description: | HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways. |
| Legal Information: | HepaRG is a trademark of BioPredic International company |
| Legal Information: | These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin. |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12352207 |