HepaRG™ Cells BCRP (-/-)
SIGMA/MTOX1021 - one vial
Product Type: Chemical
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| biological source | human female liver (hepatocarcinoma and hepatitis C tumor) |
| form | liquid |
| OMIM accession no. | 603756   |
| Quality Level | 200 |
| storage temp. | −196°C |
| Analysis Note: | Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation. |
| Application: | See technical bulletin for detailed protocols |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Features and Benefits: | HepaRGcells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG BCRP Knockout (KO) allows investigations of drug-transporter interactions involving BCRP in the liver. |
| Features and Benefits: | Zinc finger nucleases (ZFN) mediated Knockout of ABCG2 (BCRP) gene. • The frame-shift mutation of ABCG2 gene was confirmed by fragment length analysis and DNA sequencing. • Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay. |
| General description: | HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG BCRP knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways. |
| Legal Information: | Exhibit 1: ADME/Tox cell lines license Exhibit 2: HepaRG limited use license |
| Legal Information: | HepaRG is a trademark of BioPredic International company |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352207 |