MRP3 Knockout Caco-2 Cells
SIGMA/MTOX1093 - one vial
Product Type: Chemical
Caco-2 cells in culture : Bright field images of Caco-2 cells cultured on conventional 2D cell culture plates. Image shows cells at medium (left) and high density (right).
| application(s) | ADME/TOX |
| biological source | human male colorectal tissue (Source Disease: colon adenocarcinoma) |
| form | liquid |
| Quality Level | 200  |
| storage temp. | −196°C |
| technique(s) | drug transporter assay: suitable |
| permeability assay: suitable |
| Application: | The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays: Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes Caco-2 Transporter Knockout Cell Based Assays |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Features and Benefits: | The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include: • A functional knockout of the MRP3 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP3 substrate • The vial format enables the MRP3 knockout cells to be included in standard drug transporter protocols • Human assay with no interference from animal inhibitors • Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality |
| General description: | The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP3 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC3 (MRP3) efflux transporter. |
| Legal Information: | ADME/Tox Cell Lines License |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |