Canine MDR1 Knockout, Human BCRP Knockin MDCKII Cells
SIGMA/MTOX1304
Synonym: MDCKII MDR1 KO hBCRP knockin
Product Type: Chemical
| biological source | canine kidney (cocker spaniel) |
| form | liquid |
| Quality Level | 200  |
| storage temp. | −196°C |
| Analysis Note: | Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification. |
| Application: | See technical bulletin for detailed protocols |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Features and Benefits: | MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. |
| Features and Benefits: | The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human BCRP has been transfected into the MDCKII cell line. Validation studies have shown efflux of standard BCRP substrates. |
| Legal Information: | Exhibit 1: ADME/Tox cell lines license MDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352207 |