CypExpress™ 3A4 Cytochrome P450 human
SIGMA/MTOXCE3A4
Synonym: CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450 3A4; P450C3; P450PCN1
EC Number: 232-387-9
Product Type: Chemical
Cypexpress™ includes all of the critical enzymes, cofactors and antioxidants. Just add buffer to rapidly screen for drug metabolites.
| application(s) | cell analysis |
| biological source | human |
| form | dry powder |
| recombinant | expressed in Pichia pastoris |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P08684   |
| Application: | Poster: A Novel, Robust Recombinant Human P450 Biocatalytic System Poster: Evaluation of Dry Powder Preparations of Permeabilized Pichia pastoris Cells Expressing Human Cytochrome P450 2D6 Enzyme (CYP2D6) for Drug Metabolite Generation Poster: Facile Scale-up Biocatalytic Production of P-450 Metabolites Using a Novel Recombinant Human Catalytic System Poster: Multi-Cycle Production of Metabolites with human P450 systems Poster: Rapid identifcation and production of metabolites using CypExpress™ 2D6m 3A4 and 2C9 Poster: Rapid identifcation and production of metabolites using stabilized dried powder (SDP) of human cytochrome P450S (hCYPS) 2D6, 3A4, and 2C9 engineered in yeast Poster: Utilizing a Novel Biocatalytic Human P450 System for the Multi - Cycle Production of Metabolites |
| Biochem/physiol Actions: | Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. Ketoconazole, itraconazole, azamulin, troleandomycin and verapamil are inhibitors, whereas rifampin is an inducer of the enzyme. The isozyme CYP3A4 is a major pathway for the invivo conversion of testosterone to 6β-Hydroxytestosterone via hydroxylation. |
| Biochem/physiol Actions: | Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds like xenobiotics, pollutants and drug molecules. Cytochrome P450 3A4 (CYP3A4) is one of the isoforms of cytochrome P450 (P450s). CYP3A4 oxidizes more than 90% of marketed drugs through various metabolic processes. |
| Features and Benefits: | CypExpress™ 3A4 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 3A4 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress™ are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress™ retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems. It is designed for rapid screening of drug metabolites and for scaling up metabolite generation. |
| Legal Information: | 6538 is a trademark of American Type Culture Collection |
| Legal Information: | CypExpress is a trademark of Oxford Biomedical Research |
| Other Notes: | A reaction mixture containing 500 μM testosterone and 100 mg/mL of CypExpress™ 3A4 will produce 121.9 μM 6b-hydroxytestosterone in 3 hours at 30°C under the conditions described. |
| Physical form: | Light yellow, very fine powder |
| Preparation Note: | Per Protocol/Variable |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41106200 |