RPTEC Complete Supplement
SIGMA/MTOXRCSUP - sterile-filtered, suitable for cell culture, add to 500 ML of basal medium
Synonym: Basal media supplement for huRPTEC
Product Type: Chemical
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| form | liquid |
| Quality Level | 300  |
| sterility | sterile-filtered |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization. |
| General description: | Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma’s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma’s human RPTEC control SA7K Clone cells and various knockouts (KO). |
| Preparation Note: | Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |