Mix-n-Stain™ CF™ 555 Antibody Labeling Kit (5-20μg)
SIGMA/MX555S20
Product Type: Chemical
Immunocytochemistry HeLa cells stained with CF640R Mix-n-Stain labeled Mouse Anti-Tubulin antibody, red. Actin filaments are stained with CF488A phalloidin, green, and nuclei are counterstained with DAPI, blue.
Chart Absorption and emission spectra of CF555 conjugated to Goat Anti-Mouse IgG in 1× PBS buffer.
Immunohistochemistry Frozen section of Rat testis stained with Mouse Anti-tubulin and CF488A Goat Anti-Mouse (min x Rat) (microtubules, green), CF555 Mix-n-Stain labeled Mouse Anti-ZO1 (tight junctions, red) and CF640R phalloidin (actin filaments, cyan).
Immunohistochemistry Frozen section of Rat brain stained with CF555 Mix-n-Stain labeled Mouse Anti-ZO1 (red) and Mix-n-Stain CF640R labeled Mouse Anti-GFAP (cyan).
Immunohistochemistry Frozen section of Rat brain stained with CF555 Mix-n-Stain labeled Mouse Anti-ZO1 (red) and Mix-n-Stain CF640R labeled Mouse Anti-GFAP (green). Nuclei are stained with DAPI (blue).
Immunohistochemistry Frozen section of Rat brain stained with CF488A labeled Mouse Anti-NeuN (neurons, green), Rabbit-Anti-GFAP followed by CF555 Goat-Anti-Rabbit, highly cross-adsorbed (glia, red), and CF640R Mix-n-Stain labeled Mouse Anti-ZO1 (capillary tight junctions, cyan). Nuclei are counterstained with DAPI (blue).
Immunohistochemistry Frozen section of Rat brain stained with CF488A labeled Mouse Anti-NeuN (neurons, green), Rabbit-Anti-GFAP followed by CF555 Goat-Anti-Rabbit, highly cross-adsorbed (glia, red), and CF640R Mix-n-Stain labeled Mouse Anti-ZO1 (capillary tight junctions, cyan).
| fluorescence | λex 555 nm; λem 565 nm |
| form | liquid |
| Quality Level | 100  |
| storage condition | protect from light |
| storage temp. | −20°C |
| Analysis Note: | Direct replacement for: Alexa Fluor™ 555, Cy®3, DY-547, DyLight®549, TRITC |
| Application: | The Mix-n-Stain CF dye antibody labeling kit can be used to label primary antibody for ELISA, western blot, immunohistochemistry, flow cytometry, immunofluorescence or immunocytochemistry applications. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Mix-n-Stain CF dye antibody labeling kits dramatically simplify the process of preparing fluorescently labeled antibodies, particularly primary antibodies. Simply mix your antibody with a CF dye of your choice in the buffer provided, a step that takes less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have a fluorescent antibody conjugate that is as good as the best pre-labeled fluorescent antibody you can buy from some of the leading suppliers. There is no need to calculate how much dye you should use; just mix your antibody with the entire amount of dye provided and you will always produce optimal labeling. Moreover, the labeling reaction can tolerate the presence of common stabilizers, such as sodium azide, Tris, glycerol and low level of BSA. |
| General description: | This labeling kit is for labeling 5-20 μg antibody with the red fluorescent dye CF555. Mix-n-Stain CF dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF555 is an orange-red fluorescent dye and one of an outstanding series of CF dyes developed by Biotium. CF555 is spectrally similar to Alexa Fluor™555 and Cy®3 dyes. CFdyes are superior to Alexa Fluor™ dyes and Cy® dyes for antibody labeling by having combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging. |
| Legal Information: | ALEXA FLUOR is a trademark of Life Technologies |
| Legal Information: | CF is a trademark of Biotium, Inc. |
| Legal Information: | Cy is a registered trademark of Cytiva |
| Legal Information: | DyLight is a registered trademark of Pierce Biotechnology, Inc. |
| Legal Information: | Mix-n-Stain is a trademark of Biotium, Inc. |
| Legal Information: | This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer’s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com. |
| Other Notes: | The kit consists of 1 vial each of Mix-n-Stain Dye, Mix-n-Stain Reaction Buffer (10x), Mix-n-Stain Storage Buffer, and an ultrafiltration vial. |
| Hazard statements | H412 |
| Precautionary statements | P273 |
| Hazard Codes | Xn |
| Risk Statements | 22-52/53 |
| Safety Statements | 61 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |