Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse
SIGMA/N0142 - clone N52, ascites fluid
Synonym: Anti-CMT2CC; Anti-NFH
MDL Number: MFCD00164708
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Rod microglia: elongation, alignment, and coupling to form trains across the somatosensory cortex after experimental diffuse brain injury. By: Ziebell, J. M., Taylor, S. E., et al. in J Neuroinflammation, 2012. PubMed ID: 23111107 Image collected and cropped by CiteAb from the following publication, (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-247), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Schwann Cell Expressed Nogo-B Modulates Axonal Branching of Adult Sensory Neurons Through the Nogo-B Receptor NgBR. By: Eckharter, C., Junker, N., et al. in Front Cell Neurosci, 2015. PubMed ID: 26635533 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/Article/10.3389/fncel.2015.00454/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Schwann Cell Expressed Nogo-B Modulates Axonal Branching of Adult Sensory Neurons Through the Nogo-B Receptor NgBR. By: Eckharter, C., Junker, N., et al. in Front Cell Neurosci, 2015. PubMed ID: 26635533 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/Article/10.3389/fncel.2015.00454/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Frozen sections acetone fixed, paraffin-embedded Rat cerebellum tissue sections were stained with 1:400 Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in Mouse, Clone: N52 (Cat. No. N0142) followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Western Blotting Whole extract of SH-SY5Y cells was separated on SDS-PAGE and probed with Monoclonal Anti-Neurofilament 200 antibody, Clone: N52 (Cat. No. N0142). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1.1:1000 antibody 2. Negative control: without first antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | N52, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunohistochemistry (frozen sections): 1:400 using rat cerebellum | |
| microarray: suitable | |
| western blot: 1:1,000 using rat brain extract |
| Application: | Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody has been used in immunohistochemistry and western blotting. |
| Biochem/physiol Actions: | Displays a broad species cross-reactivity, recognizing both the phosphorylated and non-phosphorylated forms of this neural specific antigen using various techniques. |
| Biochem/physiol Actions: | The heavy polypeptide neurofilament encoded by neurofilament heavy chain (NEFH) gene participates in the maintenance of cytoskeleton and axonal architecture in the proximal axonal region of spinal motoneurons. Mutations in the NEFH gene has a key role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). NEFH gene is also identified to generate autosomal dominant axonal Charcot-Marie-tooth disease (CMT2cc). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Neurofilaments are composed of three subunits, namely NEFL (light), NEFM (medium) and NEFH (heavy). NEFH (neurofilament heavy) gene encodes the most important neuron-specific intermediate filament of cytoskeleton in myelinated axons. This gene is mapped to human chromosome 22q12.2. Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | C-terminal segment of enzymatically dephosphorylated pig neurofilament 200. |
| Physical form: | Supplied as ascites fluid containing 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |