Anti-Nitrotyrosine antibody produced in rabbit
SIGMA/N0409 - affinity isolated antibody, buffered aqueous solution
Synonym: Nitrotyrosine Antibody; Nitrotyrosine Antibody - Anti-Nitrotyrosine antibody produced in rabbit
MDL Number: MFCD01322409
Product Type: Chemical
Immunohistochemistry Staining of methacarn-fixed, formic acid-treated Alzheimer′s Disease (AD) brain sections, with 1:1,000 Rabbit Anti-Nitrotyrosine (Cat. No. N0409) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence
A549 cells metabolically labeled with 3-nitro-L-tyrosine were fixed and permeabilized with methanol followed by aceton. Fixed cells were stained with 1:500 Rabbit Anti-Nitrotyrosine (Cat. No. N0409). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate.
A549 cells metabolically labeled with 3-nitro-L-tyrosine were fixed and permeabilized with methanol followed by aceton. Fixed cells were stained with 1:500 Rabbit Anti-Nitrotyrosine (Cat. No. N0409). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate.
Immunofluorescence A549 cells metabolically labeled with 3-nitro-L-tyrosine were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Rabbit Anti-Nitrotyrosine (Cat. No. N0409). The antibody was developed Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Tissue lysates were separated on SDS-PAGE and probed with 1:1,000 Rabbit Anti-Nitrotyrosine (Cat. No. N0409). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat Brain 2. Mouse Brain 3. Rat Spinal cord 4. Mouse Spinal cord 5. Mouse Lung
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain |
| indirect ELISA: 1:1,000 using nitrated-BSA | |
| indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine | |
| microarray: suitable | |
| western blot: 1:1,000 using nitrated-BSA |
| Application: | Anti-Nitrotyrosine antibody produced in rabbit has been used in: • immunoblotting • enzyme-linked immunosorbent assay (ELISA) • immunohistochemistry • immunofluorescence immunocytochemistry • confocal microscopy • immunofluorescent staining |
| Biochem/physiol Actions: | Anti-Nitrotyrosine recognizes nitrated proteins and 3-nitro-L-tyrosine. It does not cross-react with L-tyrosine, ρ-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine and phospho-L-tyrosine BSA conjugates. |
| Biochem/physiol Actions: | Peroxynitrite has been shown to promote the nitration of tyrosine residues in proteins, and oxidation of redox metal centers, DNA, lipids and cysteine and methionine residues in proteins, suggesting that it plays a major role in oxidative cellular damage. Peroxynitrite has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human disease, including neurological conditions such as Alzheimer′s disease, amyotrophic lateral sclerosis (ALS), in the pathogenesis of asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury. |
| Biochem/physiol Actions: | Reacts with peroxynitrite-nitrated proteins and 3-nitro-L-tyrosine. Does not crossreact with L-tyrosine, p-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine, and phospho-L-tyrosine. Binding of the antibody is blocked by 3-nitrotyrosine. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Peroxinitrite (ONOO-), a potent oxidant linked to cellular oxidative damage, is an important member of the family of reactive oxygen and nitrogen species, rapidly generated in vivo from nitric oxide and superoxide anion. Nitrotyrosine is a product of tyrosine nitration, that is facilitated by reactive nitrogen species. |
| Immunogen: | nitrated KLH (Keyhole Limpet Hemocyanin). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | Purified by affinity chromatography on a column of 3-nitro-L-tyrosine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |