Anti-Neurocan antibody, Mouse monoclonal
SIGMA/N0913 - clone 650.24, purified from hybridoma cell culture
Synonym: Anti-CSPG3; Anti-Chondroitin Sulfate Proteogycan 3
MDL Number: MFCD02263808
Product Type: Chemical
Western Blotting Neonatal Rat brain treated with chondroitinase extract were separated on SDS-PAGE and probed with Anti-Neurocan antibody, Mouse monoclonal, Clone: 650.24 (Cat. No. N0913). The antibody was developed using 1:4,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 0.1 μg/mL antibody 2. 0.2 μg/mL antibody 3. 0.4 μg/mL antibody 4. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 650.24, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 260 kDa (additional band at 160 kDa) |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 0.1-0.2 μg/mL using neonatal rat brain treated with chondroitinase. | |
| UniProt accession no. | P55067  |
| Application: | Anti-Neurocan antibody, Mouse monoclonal has been used in: • western blotting • immunofluorescence • enzyme-linked immunosorbent assay (ELISA) • immunocytochemistry • immunohistochemistry |
| Biochem/physiol Actions: | Neurocan can inhibit the growth of axons. In presence of central nervous system injuries, it also takes part in tissue repair and neural network reconstruction. |
| Biochem/physiol Actions: | Neurocan participates in the modulation of cell adhesion and migration. It exerts a negative effect on growing cerebellar axons. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Neurocan (mouse IgG1 isotype) is derived from the hybridoma 650.24 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with embryonic rat brain proteoglycans. Neurocan is a chondroitin sulfate proteoglycan (known also as CSPG3). It accounts for 20−30% of chondroitin sulfate proteoglycans in early postnatal rat brain. The mouse neurocan cDNA encodes for a 1,257-amino acid protein with a 22-amino acid signal peptide followed by an immunoglobulin-like domain and repeating motifs characteristic of the hyaluronic acid-binding region of aggregating proteoglycans. Neurocan is found in large amounts in glial scar, a primarily astrocytic structure formed after an injury in the central nervous system (CNS). |
| General description: | Neurocan is a chondroitin sulfate proteoglycan (knownalso as CSPG3) thought to be involved in the modulation of cell adhesion and migration. It accounts for 20-30% of chondroitin sulfate proteoglycans in early postnatal rat brain. |
| General description: | Neurocan is a proteoglycan which is expressed in the nervous tissue. |
| Immunogen: | embryonic rat brain proteoglycans. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |