Anti-Nup98 antibody, Rat monoclonal
SIGMA/N1038 - clone 2H10, purified from hybridoma cell culture
Synonym: Anti-Nucleoporin, 98-kD
MDL Number: MFCD09265425
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-Nup98 Clone: 2H10 (Cat. No. N1038). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa Nuclear 2. HeLa 3. Jurkat 4. SH-SY5Y 5. COS7 6. NIH-3T3 7. P19 8. NRK
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 2H10, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2cκ |
| mol wt | antigen ~98 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 0.5-1 μg/mL using HeLa nuclear extract | |
| UniProt accession no. | P52948  |
| Application: | Anti-Nup98 antibody, Rat monoclonal may be used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunocytochemistry |
| Biochem/physiol Actions: | Nucleoporin-98kDa (Nup98) is involved in the translocation of RNA and proteins across the nucleus. It is also a regulator of chromosomal segregation and gene expression. The protein also binds to other nucleoporins and is associated with acute myelogenous leukemia. |
| Biochem/physiol Actions: | Nup98 functions as a docking protein for cytosol mediated docking of import substrates. Securin protein damage caused by the anaphase-promoting complex (APC) during mitosis, is regulated by the nucleocytoplasmic transport factors Rae1 and Nup98. In mice, collective haploinsufficiency of Rae1 and Nup98 gene causes premature separation of sister chromatids and severe aneuploidy. In addition, it also leads to untimely degradation of securin. Chromosome rearrangement between the HOXA9 (homeobox A9) gene and the nucleoporin gene Nup98 has been observed in some human myeloid leukemia patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Nup98 (rat IgG2c- isotype) is derived from the hybridoma 2H10 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from WKY/NCrj rat immunized with a recombinant fragment of human Nup98. Nup96 and Nup98 are the nucleoporins that are formed by the cleavage of 186 kDa precursor protein. |
| General description: | Nucleoporin-98kDa (Nup98) is part of the nuclear pore complex. It possesses two domains and the gene encoding it is localized on human chromosome 11. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |